Original scientific paper

https://doi.org/10.5562/cca1788

HPLC Method Validation for Simultaneous Determination of Three Mycotoxins from Corn Seeds

Augustin Curticapean ; University of Medicine and Pharmacy, 38 Gh. Marinescu Street, RO-540139 Târgu-Mures, Romania
Felicia Toma ; University of Medicine and Pharmacy, 38 Gh. Marinescu Street, RO-540139 Târgu-Mures, Romania
Monica Tarcea ; University of Medicine and Pharmacy, 38 Gh. Marinescu Street, RO-540139 Târgu-Mures, Romania
Manuela Curticapean ; University of Medicine and Pharmacy, 38 Gh. Marinescu Street, RO-540139 Târgu-Mures, Romania
Victor Samarghitan ; University of Medicine and Pharmacy, 38 Gh. Marinescu Street, RO-540139 Târgu-Mures, Romania
Ioan Aurel Pop ; S.C.D.C.B. Tg-Mures, Str. Principala 1227, RO-547530 Sangeorgiu de Mures, Romania
Alin Gulea ; S.C.D.C.B. Tg-Mures, Str. Principala 1227, RO-547530 Sangeorgiu de Mures, Romania

Abstract

A new HPLC optimized method for simultaneous determination of some mycotoxins by separation
and using a Dionex UltiMate3000 modular system, with multichannel UV detector was developed
and validated. The method optimization was performed to determine simultaneously, several relevant mycotoxins
from corn seeds that were stored over 8 months. The mycotoxins selected for quantification
were: aflatoxin B1, ochratoxin A and zearalenone. They were selected due to their high concentration in
the fodder. For the development stages of the HPLC method, it was introduced an internal standard to
have accurate results. Taking into account that the analytes should be extracted from the complex matrix
they reside, an extraction procedure was performed, using organic solvents, and the selection after
repeated tests demonstrated the best capacity for ethyl acetate. The recovery is about 60–70 % after the
extraction process also there is a good preconcentration (×2.5) of the analytes and the internal standard for
their quantification.
The limit of quantification (LOQ) obtained by chromatographic parameters optimization, for aflatoxin
B1 and ochratoxin A were about 3–5 μg kg–1 and 14.4 μg kg–1 for zearalenone of raw biological
material, making these values lower than those accepted by the actual normatives and regulations.
(doi: 10.5562/cca1788)

Keywords

method validation; mycotoxins; corn seeds analysis

Hrčak ID:

74806

URI

https://hrcak.srce.hr/74806

Publication date:

13.11.2011.

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