Original scientific paper

Avian influenza surveillance in backyard flocks and migratory birds in Slovenia

Jožko Račnik
Uroš Krapež
Tomi Trilar
Alenka Dovč
Darja Barlič- Maganja
Olga Zorman Rojs

Abstract

Blood samples and clocal swabs were taken from free-living migratory birds and backyard flock birds. Enzyme linked immunosorbent assays (ELISA) and haemagglutination inhibition test (HI) was used to screen free-living migratory birds and backyard flocks for antibodies against avian influenza viruses (AIV). Pools of cloacal swabs were used for the attempt of isolation of AIV. Samples were inoculated of 9 to 11 day-old chicken embryos. Amnioallantoic fluid were collected and analysed for haemagglutination activity. Pools of cloacal swabs were used for the detection of AIV by RT-PCR. Sera samples of four out of twenty three geese were positive on presence of specific antibodies against AIV. All attempts of virus isolation were negative. No AIV nucleic acid was detected by RT-PCR.

Keywords

Migratory birds; Backyard flocks; Avian influenza; Seropre-valence; RT-PCR

Hrčak ID:

7746

URI

https://hrcak.srce.hr/7746

Publication date:

25.11.2006.

Article data in other languages: croatian

Visits: 1.571 *