Original scientific paper
Comparison and evaluation of different methodologies and tests for detection of antidsDNA antibodies on 889 Slovenian patients’ and blood donors’ sera
Polona Žigon
; University Medical Centre Ljubljana, Department of Rheumatology, Immunology Laboratory, Ljubljana, Slovenia
Katja Lakota
; University Medical Centre Ljubljana, Department of Rheumatology, Immunology Laboratory, Ljubljana, Slovenia
Saša Čučnik
; University Medical Centre Ljubljana, Department of Rheumatology, Immunology Laboratory, Ljubljana, Slovenia
Tinka Švec
; University Medical Centre Ljubljana, Department of Rheumatology, Immunology Laboratory, Ljubljana, Slovenia
Aleš Ambrožič
; University Medical Centre Ljubljana, Department of Rheumatology, Immunology Laboratory, Ljubljana, Slovenia
Snežna Sodin-Šemrl
; University Medical Centre Ljubljana, Department of Rheumatology, Immunology Laboratory, Ljubljana, Slovenia
Tanja Kveder
; University Medical Centre Ljubljana, Department of Rheumatology, Immunology Laboratory, Ljubljana, Slovenia
Abstract
Aim To evaluate four different commercially available assays
for anti-double stranded DNA (dsDNA) detection and
compare them with the in-house radioimmunoassay according
to Farr (FARR-RIA) in order to select the optimal primary
method for use in combination with FARR-RIA.
Methods Sera from 583 consecutive patients sent to our
laboratory for routine diagnosis, 156 selected patients with
autoimmune diseases (76 systemic lupus erythematosus
[SLE] patients and 80 patients with other autoimmune diseases),
and 150 blood donors were tested for anti-dsDNA
antibodies with two enzyme-linked immunoassays (ELISA),
two Crithidia luciliae immunoflourescence tests (CLIFT),
and FARR-RIA. The specificities and sensitivities of the tests
were calculated and compared.
Results FARR-RIA and CLIFT 2 showed the highest specificity
for SLE (100%), with CLIFT 2 showing higher sensitivity
(33% vs 47%). Both ELISAs showed higher sensitivities
(>53%) than FARR-RIA but lower specificities (<93%),
whereas CLIFT 1 showed the lowest overall agreement
with FARR-RIA.
Conclusion CLIFT 2 was selected as the primary test for
use in combination with FARR-RIA. The use of CLIFT 2 reduced
the number of sera that needed to be tested by
FARR-RIA, the time needed to report the results, and environmental
toxicity, cancerogenicity, and radioactivity.
Keywords
anti-dsDNA antibodies; FARR assay; Crithidia luciliae; systemic lupus erythematosus, enzyme immunoassay; IIF
Hrčak ID:
78199
URI
Publication date:
15.12.2011.
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