Original scientific paper
Haplotype frequencies of 17 Y-chromosomal short tandem repeat loci from the Cukurova region of Turkey
Ayse Serin
; Cukurova University Medical Faculty, Department of Forensic Medicine, Adana, Turkey
Husniye Canan
; Cukurova University Medical Faculty, Department of Forensic Medicine, Adana, Turkey
Behnan Alper
; Cukurova University Medical Faculty, Department of Forensic Medicine, Adana, Turkey
Yasar Sertdemir
; Cukurova University Medical Faculty, Department of Biostatistics, Adana, Turkey
Abstract
Aim To investigate the distribution of 17 Y-short tandem
repeat (STR) loci in the population of the Cukurova region
of Turkey.
Methods In the period between 2009 and 2010, we investigated
the distribution of 17 Y-STRs in a sample of 249
unrelated healthy men from the Cukurova region of Turkey.
Genomic DNA was extracted with InstaGene matrix
and Y-STRs were determined using the AmpFISTR Yfiler
PCR amplification kit. Gene and haplotype diversity values
were estimated using the Arlequin software. To compare
our data to other populations, population pairwise genetic
distances and associated probability values were calculated
using the Y Chromosome Haplotype Reference Database
Web site software.
Results At 17 Y-STR loci we detected 148 alleles. The lowest
gene diversity in this region was 0.51 for DYS391 and
the highest 0.95 for DYS385a/b. Haplotype diversity was
0.9997 ± 0.0004. We compared our data with haplotype data
of other Turkish populations and no significant differences
were found, except with Ankara population (Φst = 0.025,
P = 0.018). Comparisons were also made with the neighboring
populations using analysis of molecular variance
of the Y-STR loci genetic structure and our population was
nearest to Lenkoran-Azerbaijani (Φst = 0.012, P = 0.068) and
Iranian Ahvaz population (Φst = 0.007, P = 0.173), followed
by Greek (Φst = 0.026, P = 0.000) and Russian (Φst = 0.048,
P = 0.000) population. Other countries like Portugal, Spain,
Italy, Egypt, Israel (Palestinian Authority Area), and Taiwan
showed a high genetic distance from our population.
Conclusion Our study showed that Y-STR polymorphisms
were a powerful discrimination tool for routine forensic
applications and could be used in genealogical investigations.
Keywords
Y-STRs; population studies; Turkey population; haplotype frequencies; genealogical investigation
Hrčak ID:
78200
URI
Publication date:
15.12.2011.
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