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Archives of Industrial Hygiene and Toxicology, Vol. 63 No. 1, 2012.

Original scientific paper

Cytotoxic Effects of Imidazolium Ionic Liquids on Fish and Human Cell Lines

Marina Cvjetko ; Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia
Kristina Radošević ; Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia
Andrej Tomica ; Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia
Igor Slivac ; Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia
Jasna Vorkapić-Furač ; Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia
Višnja Gaurina Srček orcid id orcid.org/0000-0001-6591-3810 ; Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia

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Abstract

Ionic liquids bring a promise of a wide range of “green” applications that could replace conventional volatile solvents. However, before these applications become large-scale, their toxicity needs to be investigated in order to predict the impact on human health and environment. In this study we assessed the cytotoxicity of imidazolium ionic liquids (in the concentrations between 0.1 mmol L-1 and 10 mmol L-1) in the ovarian fi sh cell line CCO and the human tumour cell line HeLa using the MTT cell viability assay. Our results showed that the most cytotoxic ionic liquid was 1-n-butyl-3-methylimidazolium bis(trifluoro methylsulphonyl)imide, [BMIM][Tf2N], followed by 1-n-butyl-3-methylimidazolium tetrafl uoroborate [BMIM][BF4], 1-n-butyl-3-methylimidazolium hexafluorophosphate [BMIM][PF6], and 1,3-dimethylimidazolium hexafl uorophosphate [MMIM][PF6]. Generally, the effects were concentrationdependent. They also depended on the type of anion and the n-alkyl chain length. The comparison between the fish CCO and human HeLa cell lines suggests that CCO cells provide a good biological system for initial toxicity testing of ionic liquids that could replace in vivo bioassays.

Keywords

CCO cells; HeLa cells; imidazolium ionic liquids; MTT assay

Hrčak ID:

78632

URI

https://hrcak.srce.hr/78632

Publication date:

21.3.2012.

Article data in other languages: croatian

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