Short communication, Note
https://doi.org/10.2478/10004-1254-63-2012-2114
Effects of Taurine on Permethrin-Induced Cytogenetic and Oxidative Damage in Cultured Human Lymphocytes
Hasan Turkez
; Erzurum Technical University, Faculty of Science, Department of Molecular Biology and Genetics, Erzurum, Turkey
Elanur Aydin
; Atatürk University, Faculty of Science, Department of Biology, Erzurum, Turkey
Abstract
Permethrin (PM) is a common pyrethroid pesticide used to control pests in agriculture, forestry, horticulture, health care, homes, and textile industry. It is confi rmed as a strong mutagen in animals and humans. Taurine (TA) is an amino acid found in mammalian tissues that protects the cell against DNA damage. In this study, we investigated whether supplementation of human lymphocyte cultures with TA (in the concentrations of 25 μg mL-1, 50 μg mL-1 and 100 μg mL-1) provided any protection against PM toxicity applied in the concentration of 200 μg mL-1. Genotoxicity was assessed using the micronucleus (MN) and sister chromatid exchanges (SCE) tests. In addition, we measured the total antioxidant capacity (TAC) and total oxidative stress (TOS) levels in the plasma to determine oxidative effects. PM increased SCE and MN levels and altered TAC and TOS levels. TA alone did not affect SCE and MN levels compared to controls, regardless of the concentration applied. In addition, it increased TAC levels without changing TOS levels. Moreover, it signifi cantly buffered the negative cytogenetic and oxidative effects induced by PM in a clear dose-dependent manner. In conclusion, this study is the first to evidence the beneficial effects of TA against PM-induced DNA and oxidative damages in vitro.
Keywords
antioxidant; micronucleus; pesticide; sister chromatid exchanges
Hrčak ID:
78636
URI
Publication date:
21.3.2012.
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