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Acta Pharmaceutica, Vol. 62 No. 2, 2012.

Original scientific paper

https://doi.org/10.2478/v10007-012-0015-0

Development and validation of a bioanalytical LC-UV method with solid-phase extraction for determination of valproic acid in saliva

JASMINA TONIC-RIBARSKA orcid id orcid.org/0000-0002-0026-3252 ; Institute of Applied Chemistry and Pharmaceutical Analysis, Faculty of Pharmacy, University “Ss Cyril and Methodius”, Skopje, Macedonia
ARLINDA HAXHIU ; Department of Pharmacy, Medical Sciences Faculty, State University of Tetova, Tetovo, Macedonia
ZORAN STERJEV ; Institute of Pharmaceutical Chemistry, Faculty of Pharmacy, University “Ss Cyril and Methodius”, Skopje, Macedonia
GORDANA KITEVA ; Clinic of Neurology, Faculty of Medicine, University “Ss Cyril and Methodius”, Skopje, Macedonia
LjUBICA SUTURKOVA ; Institute of Pharmaceutical Chemistry, Faculty of Pharmacy, University “Ss Cyril and Methodius”, Skopje, Macedonia
SUZANA TRAJKOVIC-JOLEVSKA ; Institute of Applied Chemistry and Pharmaceutical Analysis, Faculty of Pharmacy, University “Ss Cyril and Methodius”, Skopje, Macedonia

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Abstract

A bioanalytical HPLC method with UV detection for the determination of the antiepileptic drug valproic acid in human saliva has been developed and validated. Saliva represents an alternative matrix for monitoring of antiepileptic drugs due to the increasing interest in free drug concentration. The proposed method involved solid-phase extraction for sample preparation and yielded very good mean recoveries of 99.4 % and 97.9 % for valproic acid and IS, respectively. The calibration function for valproic acid was linear over the concentration range of 1.0–50.0 µg mL1 (R2 = 0.9989). Within-run and between-run precision and accuracy were studied at four concentrations and RSDs were less than 7.3 and 2.2 %, while accuracy values were higher than 96.8 and 97.5 %, respectively. The described method provides sensitivity, linearity, precision, accuracy and is suitable for analyses of valproic acid in saliva samples.

Keywords

valproic acid; saliva; solid-phase extraction; high-performance liquid chromatography; validation

Hrčak ID:

78866

URI

https://hrcak.srce.hr/78866

Publication date:

30.6.2012.

Article data in other languages: croatian

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