Original scientific paper

Conventional isolation and polymerase chain reaction assay for detection of Escherichia coli O157:H7 from intestines of philippine bats.

Jomalyn T. Italia ; Department of Veterinary Paraclinical Sciences, College of Veterinary Medicine, University of the Philippines Los Baños, College Laguna, Philippines
Hope G. Rovira ; Department of Veterinary Paraclinical Sciences, College of Veterinary Medicine, University of the Philippines Los Baños, College Laguna, Philippines
Joseph S. Masangkay ; Department of Veterinary Paraclinical Sciences, College of Veterinary Medicine, University of the Philippines Los Baños, College Laguna, Philippines
Yasuhiro Yoshikawa ; University of Tokyo, Graduate School of Agricultural and Life Sciences, Japan
Maria Theresa M. Perez ; National Institute of Molecular Biology and Biotechnology, University of the Philippines Los Baños, College Laguna, Philippines
Alisha Wehdnesday B. Reyes ; Department of Veterinary Paraclinical Sciences, College of Veterinary Medicine, University of the Philippines Los Baños, College Laguna, Philippines
Waren N. Baticados ; Department of Veterinary Paraclinical Sciences, College of Veterinary Medicine, University of the Philippines Los Baños, College Laguna, Philippines

Abstract

It is currently reported that bats in the Philippines harbor bacterial organism (Salmonella spp.) with pathogenic potential. The paper describes the conventional isolation and polymerase chain reaction (PCR) assay for the detection of another bacterium, Escherichia coli, from a sample population of 56 apparently healthy bats collected from Laguna and Quezon City, Philippines. Nineteen of the samples were positive for E. coli using the conventional method of isolation, while PCR molecularly detected the bacteria in 15 samples. The presence of hemolysin among the isolates was not observed. The isolates were subjected to E. coli O157:H7 serotype detection using the latex agglutination test and another PCR assay specific for this serotype. The data revealed that none of the isolates was positive for E. coli O157:H7 using serological and molecular diagnostic methods, which indicates that bats from Laguna and Quezon City, Philippines were not carriers of the pathogenic strain, E. coli O157:H7. The study also presents the first local report of conventional isolation and molecular detection of E. coli from Philippine bats.

Keywords

Escherichia coli; latex agglutination test; Philippine bats; polymerase chain reaction

Hrčak ID:

80693

URI

https://hrcak.srce.hr/80693

Publication date:

27.4.2012.

Article data in other languages: croatian

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