Original scientific paper

The effects of different syringe volume, needle size and sample volume on blood gas analysis in syringes washed with heparin

Tuncay Küme orcid.org/0000-0003-3440-3513
Ali Rıza Şişman ; Dokuz Eylul University Medical School, Department of Medical Biochemistry, Izmir, Turkey
Ahmet Solak ; Ipekyolu State Hospital, Biochemistry Laboratory, Van, Turkey
Birsen Tuğlu ; Dr. Behçet Uz Children Hospital, Biochemistry Laboratory, İzmir, Turkey
Burcu Çinkooğlu ; Dokuz Eylul University Medical School, Department of Medical Biochemistry, Izmir, Turkey
Canan Çoker ; Dokuz Eylul University Medical School, Department of Medical Biochemistry, Izmir, Turkey

Abstract

Introductıon: We evaluated the effect of different syringe volume, needle size and sample volume on blood gas analysis in syringes washed with heparin.
Materials and methods: In this multi-step experimental study, percent dilution ratios (PDRs) and final heparin concentrations (FHCs) were calculated by gravimetric method for determining the effect of syringe volume (1, 2, 5 and 10 mL), needle size (20, 21, 22, 25 and 26 G) and sample volume (0.5, 1, 2, 5 and 10 mL). The effect of different PDRs and FHCs on blood gas and electrolyte parameters were determined. The erroneous results from nonstandardized sampling were evaluated according to RiliBAK’s TEa.
Results: The increase of PDRs and FHCs was associated with the decrease of syringe volume, the increase of needle size and the decrease of sample volume: from 2.0% and 100 IU/mL in 10 mL-syringe to 7.0% and 351 IU/mL in 1 mL-syringe; from 4.9% and 245 IU/mL in 26G to 7.6% and 380 IU/mL in 20 G with combined 1 mL syringe; from 2.0% and 100 IU/mL in full-filled sample to 34% and 1675 IU/mL in 0.5 mL suctioned sample into 10 mL-syringe. There was no statistical difference in pH; but the percent decreasing in pCO2, K+, iCa2+, iMg2+; the percent increasing in pO2 and Na+ were sta-tistical significance compared to samples full-filled in syringes. The all changes in pH and pO2 were acceptable; but the changes in pCO2, Na+, K+ and iCa2+ were unacceptable according to TEa limits except fullfilled-syringes.
Conclusions: The changes in PDRs and FHCs due nonstandardized sampling in syringe washed with liquid heparin give rise to erroneous test results for pCO2 and electrolytes.

Keywords

blood gas analysis; blood specimen collection; heparin; needles; syringes

Hrčak ID:

83224

URI

https://hrcak.srce.hr/83224

Publication date:

15.6.2012.

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