Original scientific paper

https://doi.org/10.5562/cca1810

An Overexpression of Icam-1 in Mild Hyperhomocysteinemia and Hyperglycemia − A Study of Antidiabetics Administration Effect

Tatjana Bačun orcid.org/0000-0001-7012-5325 ; J. J. Strossmayer University of Osijek, School of Medicine, University Hospital Centre Osijek, Huttlerova 4, HR-31000 Osijek, Croatia;J. J. Strossmayer University of Osijek, School of Medicine, Internal Medicine Clinic, Huttlerova 4, HR-31000 Osijek, Croa
Tatjana Belovari ; J. J. Strossmayer University of Osijek, School of Medicine, University Hospital Centre Osijek, Huttlerova 4, HR-31000 Osijek, Croatia
Aleksandar Včev ; J. J. Strossmayer University of Osijek, School of Medicine, University Hospital Centre Osijek, Huttlerova 4, HR-31000 Osijek, Croatia;J. J. Strossmayer University of Osijek, School of Medicine, Internal Medicine Clinic, Huttlerova 4, HR-31000 Osijek, Croa
Ivan Mihaljević ; J. J. Strossmayer University of Osijek, School of Medicine, University Hospital Centre Osijek, Huttlerova 4, HR-31000 Osijek, Croatia;J. J. Strossmayer University of Osijek, School of Medicine, Clinical Institute of Nuclear Medicine and Radiation Protecti
Toni Hanich orcid.org/0000-0002-8687-0933 ; J. J. Strossmayer University of Osijek, School of Medicine, University Hospital Centre Osijek, Huttlerova 4, HR-31000 Osijek, Croatia
Vladimir Fijačko ; J. J. Strossmayer University of Osijek, School of Medicine, Internal Medicine Clinic, Huttlerova 4, HR-31000 Osijek, Croatia
Ljubica Glavaš-Obrovac orcid.org/0000-0001-7497-296X ; J. J. Strossmayer University of Osijek, School of Medicine, University Hospital Centre Osijek, Huttlerova 4, HR-31000 Osijek, Croatia;J. J. Strossmayer University of Osijek, School of Medicine, Internal Medicine Clinic, Huttlerova 4, HR-31000 Osijek, Croa

Abstract

Elevated plasma homocysteine is connected to atherosclerosis and increased cerebrovascular
and ischemic heart disease especially in the patients with type 2 diabetes. Effects of metformin (10 μg
mL−1), insulin (1 mUI mL−1), and their combination administration in condition of mild
hyperhomocysteinemia (30 μmol dm−3) and hyperglycemia (12 mmol dm−3) on human aortic endothelial
cells (HAEC) was investigated. HAEC were cultured 4 h in a medium with homocysteine (7 and 30 μmol
dm−3) and glucose (5.5 and 12.0 mmol dm−3). E-selectin, intercellular adhesion molecule-1 (ICAM-1), and
vascular adhesion molecule-1 (VCAM-1) expressions were analysed by flow-cytometry. Controls were
CAMs expression on HAEC in medium with physiological concentration of homocysteine and glucose.
HAEC, incubated additionally with 30 μmol dm−3 homocysteine, increased expression of ICAM-1 for 65
% (p = 0.07) and decreased of E-selectin for 30 % (p = 0.07) and VCAM-1 for 13 % (0.06). In condition
of mild hyperhomocysteinemia and hyperglycemia there was a statistically significant increase of ICAM-
1 expression (for 22 %, p = 0.04), while administration of metformin and insulin did not statistically significantly
influence adhesion molecules expression. Observed significant overexpression of ICAM-1,
leads us to conclude that combination of mild hyperhomocisteinemia and hyperglycemia could have significant
influence on development of inflammation and atherosclerosis in diabetic patients.(doi:
10.5562/cca1810)

Keywords

Cell adhesion molecules; human aortic endothelial cells; mild hyperhomocysteinemia; mild hyperglycemia; metformin; insulin

Hrčak ID:

84531

URI

https://hrcak.srce.hr/84531

Publication date:

11.5.2012.

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