Original scientific paper

https://doi.org/10.5562/cca1816

Early Endosomal Retention of Murine Cytomegalovirus m06 Protein

Natalia Kučić ; Department of Physiology and Immunology, Medical Faculty, University of Rijeka, Braće Branchetta 20, HR-51000 Rijeka, Croatia
Maja Ilić Tomaš ; Department of Physiology and Immunology, Medical Faculty, University of Rijeka, Braće Branchetta 20, HR-51000 Rijeka, Croatia
Hana Mahmutefendić ; Department of Physiology and Immunology, Medical Faculty, University of Rijeka, Braće Branchetta 20, HR-51000 Rijeka, Croatia
Gordana Blagojević ; Department of Physiology and Immunology, Medical Faculty, University of Rijeka, Braće Branchetta 20, HR-51000 Rijeka, Croatia
Pero Lučin ; Department of Physiology and Immunology, Medical Faculty, University of Rijeka, Braće Branchetta 20, HR-51000 Rijeka, Croatia

Abstract

Murine cytomegalovirus (MCMV) encodes several genes which products interact with MHC
class I molecules (MHC-I) in the secretory pathway to evade antigen presentation. The product of
MCMV-m06 gene associates with nascent MHC-I proteins and redirects them into lysosomes for degradation.
This report will demonstrate that MCMV-m06 protein is retained in perinuclear early endosomes together
with internalized MHC-I molecules. Since we were not able to capture the MCMV-m06 protein on
the cell surface, our data suggests that the MCMV-m06 protein is transported from the Golgi into early
endosomal intermediates prior to their maturation into multivesicular endosomes and lysosomes. In NIH
3T3 cells with the transfected m06 gene, the endosomal localization of the m06 protein was not observed,
suggesting that another MCMV function remodels the route of m06 trafficking from the Golgi towards
late endosomes. (doi: 10.5562/cca1816)

Keywords

murine cytomegalovirus; m06/gp48; major histocompatibility class I molecules; early endosomal retention

Hrčak ID:

84540

URI

https://hrcak.srce.hr/84540

Publication date:

11.5.2012.

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