Original scientific paper

Isolation of Neospora caninum from an aborted fetus of seropositive cattle in Iran.

Nasrin Salehi ; Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
Hamidreza Haddadzadeh ; Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Parviz Shayan ; Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Mohammad K. Koohi ; Department of Basic Science, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

Abstract

Neospora caninum was isolated from the brain of aborted 4-month-old bovine fetuses in Tehran, Iran. From Neospora seropositive dairy cattle, cerebral tissues from aborted fetuses were inoculated interaperinoneally into immunosuppressed Balb/C mice. After 60 days, positive ELISA titer for Neospora caninum was determined in one group of mice, which had been inoculated with the brain of the 4-month-old aborted fetus and Neospora tachyzoite-like organisms were detected in the peritoneal exudates of the mice. Neospora caninum tachyzoites were isolated in Vero cell culture after inoculation with mice peritoneal exudates within 37 days. The identity of the parasite was confirmed using the nested-PCR method in the brain tissue of the aborted calf, peritoneal exudates and brains of the positive mice and cell culture. The nucleotide sequence from the brain and peritoneal exudate of the inoculated mice was designated as Nc-Iran and has been deposited in the GenBank database under the accession number FJ655914. This is the first report dealing with the isolation of N. caninum in Iran.

Keywords

Neospora caninum; cattle; brain; mice; cell culture; Iran

Hrčak ID:

93347

URI

https://hrcak.srce.hr/93347

Publication date:

5.12.2012.

Article data in other languages: croatian

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