Original scientific paper

https://doi.org/10.2478/10004-1254-63-2012-2206

Control of T-2 Toxin in Fusarium langsethiae and Geotrichum candidum Co-Culture

Elida Gastélum-Martínez orcid.org/0000-0003-1629-2135 ; Université de Toulouse, INPT-ENSAT, Laboratoire de Génie Chimique Castanet-Tolosan, Toulouse, France
Stephane Compant ; Université de Toulouse, INPT-ENSAT, Laboratoire de Génie Chimique Castanet-Tolosan, Toulouse, France
Patricia Taillandier orcid.org/0000-0003-2106-6490 ; Université de Toulouse, INP-ENSIACET, Laboratoire de Génie Chimique, CNRS, Laboratoire de Génie Chimique, Toulouse, France
Florence Mathieu ; Université de Toulouse, INPT-ENSAT, Laboratoire de Génie Chimique Castanet-Tolosan, Toulouse, France

Abstract

Due to contamination of barley grains by Fusarium langsethiae, T-2 toxin can be present in the brewing process. It has been observed that the presence of the yeast Geotrichum candidum during malting can reduce the final concentration of this mycotoxin in beer. In this work, a co-culture method was carried out for both microorganisms in order to evaluate the effect on T-2 mycotoxin concentration in comparison with the pure culture of F. langsethiae in the same conditions. The microbial growth of both microorganisms was assessed using three different methods: dry weight, DOPE-FISH, and DNA quantifi cation. In coculture, both microorganisms globally developed less than in pure cultures but G. candidum showed a better growth than F. langsethiae. The concentration of T-2 was reduced by 93 % compared to the pure culture. Hence, the interaction between G. candidum and F. langsethiae led to a drastic mycotoxin reduction despite the only partial inhibition of fungal growth.

Keywords

brewing; contamination; growth of microorganisms; mycotoxins

Hrčak ID:

93647

URI

https://hrcak.srce.hr/93647

Publication date:

14.12.2012.

Article data in other languages: croatian

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