Original scientific paper

https://doi.org/10.5562/cca2108

High Resolution Structure of the Manganese Derivative of Insulin

Biserka Prugovečki ; Division of General and Inorganic Chemistry, Department of Chemistry, Faculty of Science, University of Zagreb, Horvatovac 102a, HR-10000 Zagreb, Croatia
Ivana Pulić ; Division of General and Inorganic Chemistry, Department of Chemistry, Faculty of Science, University of Zagreb, Horvatovac 102a, HR-10000 Zagreb, Croatia
Martina Toth ; Division of General and Inorganic Chemistry, Department of Chemistry, Faculty of Science, University of Zagreb, Horvatovac 102a, HR-10000 Zagreb, Croatia
Dubravka Matković-Čalogović ; Division of General and Inorganic Chemistry, Department of Chemistry, Faculty of Science, University of Zagreb, Horvatovac 102a, HR-10000 Zagreb, Croatia

Abstract

The structure of the human manganese derivative of insulin was determined using the single crystal X-ray diffraction method. Single crystals were grown by the hanging drop vapour diffusion
crystallization method using zinc-free insulin and manganese(II) sulphate monohydrate in citrate buffer at pH = 6.4. It crystallizes in the trigonal system in the space group R3 with the unit cell parameters: a = b = 81.70 Å and c = 33.73 Å. There are two manganese ions per insulin hexamer in the human manganese-insulin derivative. The manganese ions lie on the three-fold crystallographic axis on opposite sides of
the hexamer. Both manganese ions are octahedrally coordinated by three Nε2 atoms from the imidazolyl side chains of three symmetry-related HisB10/HisD10 and three oxygen atoms of three symmetry-related water molecules. The investigated human manganese-insulin derivative adopts the T6 conformation.
(doi: 10.5562/cca2108)

Keywords

insulin derivative; manganese; X-ray structure

Hrčak ID:

93731

URI

https://hrcak.srce.hr/93731

Publication date:

17.12.2012.

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