Original scientific paper

https://doi.org/10.5562/cca2106

Surface-enhanced Raman Scattering Study of the Binding Modes of a Dibenzotetraaza[14]annulene Derivative with DNA/RNA Polynucleotides

Snežana Miljanić orcid.org/0000-0003-1179-2253 ; Laboratory of Analytical Chemistry, Department of Chemistry, Faculty of Science, University of Zagreb, Horvatovac 102a, HR-10000 Zagreb, Croatia
Adriana Dijanošić ; Laboratory of Analytical Chemistry, Department of Chemistry, Faculty of Science, University of Zagreb, Horvatovac 102a, HR-10000 Zagreb, Croatia
Matea Kalac ; Laboratory of Analytical Chemistry, Department of Chemistry, Faculty of Science, University of Zagreb, Horvatovac 102a, HR-10000 Zagreb, Croatia
Marijana Radić Stojković ; Laboratory for Study of Interactions of Biomacromolecules, Division of Chemistry and Biochemistry, Ruđer Bošković Institute, P.O.B. 1016, HR-10000 Zagreb, Croatia
Ivo Piantanida ; Laboratory for Study of Interactions of Biomacromolecules, Division of Chemistry and Biochemistry, Ruđer Bošković Institute, P.O.B. 1016, HR-10000 Zagreb, Croatia
Dariusz Pawlica ; Department of Chemistry, Jagiellonian University, Ingardena 3, 30-060 Krakow, Poland
Julita Eilmes ; Department of Chemistry, Jagiellonian University, Ingardena 3, 30-060 Krakow, Poland

Abstract

Binding modes of a dibenzotetraaza14annulene (DBTAA) derivative with synthetic nucleic acids were studied using surface-enhanced Raman spectroscopy (SERS). Changes in SERS intensity and appearance of new bands in spectra were attributed to different complexes formed between the DBTAA molecules and DNA/RNA polynucleotides. A decrease in intensity pointed to intercalation as the dominant binding mode of the annulene derivative with poly dGdC-poly dGdC and poly rA-poly rU, whereas new bands in the spectra at 735 cm−1 and 1345 cm−1 revealed binding within the minor groove of poly dAdT-poly dAdT. When all the dominant binding sites were occupied, SERS spectra implied that small molecules bind on the outside of the DNA analogues, while exist mainly as free molecules in equimolar ratio with the synthetic RNA polynucleotide, thereby indicating higher affinity for DNA than for RNA.(doi: 10.5562/cca2106)

Keywords

surface-enhanced Raman scattering; binding; polynucleotide; annulene

Hrčak ID:

94469

URI

https://hrcak.srce.hr/94469

Publication date:

17.12.2012.

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