Original scientific paper
Adaptive Changes of the Electrophoretic Mobility of Cell Nuclei (EMN) Index in the Intensive Physical Exercise of Male Rowers with Different Training Experience
Ewa Szczepanowska
; University of Szczecin, Faculty of Geosciences, Leisure Studies Unit, Szczecin, Poland
Zbigniew Czapla
; Adam Mickiewicz University, Faculty of Biology, Institute of Anthropology, Department of Human Biological Development,
Joachim Cieslik
; Adam Mickiewicz University, Faculty of Biology, Institute of Anthropology, Department of Human Biological Development,
Abstract
The aim of this study is to attempt to determine the relationship between the degree of the EMN index and the depth of changes of selected hormones and metabolic parameters as an effect of intensive physical exercise during the training process in male rowers. Juniors (N=62; chronological age 16.4 y.o. SD=1.14 y.; training experience 3.0 y.o. SD=1.05 y.) and seniors (N=27; chronological age 21.4 y. SD=1.73 y.; training experience 5.5 y.o. SD=1.10 y.), in the preparatory period of the training process, performed physical exercise of maximum intensity on a rowing ergometer. Acid-base balance parameters (pH, BE) and the concentration of lactic acid (LA) were determined as the result of physical exercise. Some selected hormones were also indicated (hGH, PrL and Prg) to show their exercise changes. A sample of buccal epithelium cells was taken from each of the male rowers, before and after the exercise, to evaluate the percentage of the EMN index by intracellular microelectrophoresis. A greater differentiation of metabolic changes during exercise was manifested in juniors
than seniors. This was reflected in changes of acid-base balance parameters, exercise physiological parameters, hormone concentration and also in changes of the EMN index. These changes were probably dependent on deep metabolic processes of an acid character during exercise. This could prove a more stable homeostasis through more economical metabolic reactions in seniors as the effect of the training process, meaning that seniors were better adapted to heavy physical exercise than juniors.
Keywords
intracellular microelectrophoresis; buccal epithelium cells; EMN index; exercise physiological parameters; male rowers; adaptive changes Introduction
Hrčak ID:
94884
URI
Publication date:
27.12.2012.
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