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Interleukin-13 Promoter Gene Polymorphism -1112 C/T Is Associated with Atopic Dermatitis in Polish Patients
Jolanta Gleń
; Department of Dermatology, Venereology and Allergology,
Magdalena Trzeciak
; Małgorzata Sokołowska-Wojdyło, MD, PhD Department of Dermatology, Venereology and Allergology Medical University of Gdańsk Dębinki 7th Str. 80-210 Gdańsk Poland
Michał Sobjanek
Tomasz Bandurski
Aleksandra Wilkowska
Bogusław Nedoszytko
Jadwiga Roszkiewicz
Małgorzata Sokołowska-Wojdyło
Abstract
Interleukin 13 (IL-13) is one of the major cytokines involved
in the IgE synthesis in patients with atopic dermatitis (AD). IL-13 gene has
been mapped on chromosome 5q31-33 region associated with atopic
conditions, where several polymorphisms are described. The aims of the
study were to establish the frequency of the IL-13 gene polymorphism and
its relation to serum IgE and IL-13 levels and SCORAD. In 180 AD patients
and 167 healthy volunteers, the -1112 C/T IL-13 gene polymorphism was
genotyped using allele-specific PCR method. Serum total IgE (tIgE) level
was measured by Uni-CAP 100 and IL-13 level using ELISA standard kit. The
-1112T allele frequency was significantly higher in AD patients compared
to controls (P=0.00001). The TT and CT genotypes were correlated with increased
serum tIgE concentration (P=0.0004). The TT genotype was associated
with severe, CT genotype with moderate and CC genotype with mild
course of AD (P=0.0008). Both CT and TT genotypes predominated in cases
of AD with concomitant asthma, while CC genotype was not found in any
case in this group (P=0.03). The mean levels of serum IL-13 and tIgE were
significantly higher in AD with concomitant asthma than in cases without
asthma (IL-13 P=0.03 and tIgE P=0.0001). There was positive correlation
between serum concentration of IL-13 (P=0.0005) and tIgE (P=0.0000001)
and severity of AD as assessed by SCORAD index. Our results confirmed
the significant role of -1112 C/T IL-13 gene polymorphism in the pathogenesis
of AD.
Keywords
atopic dermatitis; gene polymorphism; interleukin-13; SCORAD; tIgE
Hrčak ID:
95696
URI
Publication date:
11.1.2013.
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