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Archives of Industrial Hygiene and Toxicology, Vol. 64 No. 1, 2013.

Original scientific paper

https://doi.org/10.2478/10004-1254-64-2013-2274

Biological Significance of the Over-Expression of HSP70 and Alpha B-Crystallin in Rat Substantia nigra Exposed to Different Doses of Permethrin

Dilek Guvenc ; Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Ondokuz Mayis, Samsun, Turkey
Yonca Betil Kabak ; Department of Pathology, Faculty of Veterinary Medicine, University of Ondokuz Mayis, Samsun, Turkey
Enes Atmaca ; Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Ondokuz Mayis, Samsun, Turkey
Abdurrahman Aksoy ; Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Ondokuz Mayis, Samsun, Turkey
Tolga Guvenc ; Department of Pathology, Faculty of Veterinary Medicine, University of Ondokuz Mayis, Samsun, Turkey

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Abstract

The aim of this study was to investigate the possible role of the Heat Shock Protein 70 (HSP70) and Alpha B-crystallin (αBC) in the substantia nigra of rats exposed to permethrin at different doses on the apoptotic cell status. The orogastric gavage method was used to administer the different doses of permethrin (75 mg kg-1 in Group I, 150 mg kg-1 in group II, 300 mg kg-1 in group III) to the rats. Using the Western blot test, all the permethrin-treated groups showed a dose-dependent increase in the expression of HSP70 and αBC when compared to the control group. TUNEL positive apoptotic cells were not detected in the dopaminergic neurons of the substantia nigra after treatment with permethrin; however, upon immunofluorescent staining, intense positive reactions for HSP70 and αBC were observed in all of the treated groups. No immunopositive cells were detected in the tissue sections of the control group. These results suggest that the different administered doses of permethrin did not cause apoptotic cell death in the substantia nigra dopaminergic neurons; however, they did induce an increase in HSP70 and αBC expression. Thus, it appears that HSP70 and αBC could play a neuroprotective role in permethrin-induced neurotoxicity

Keywords

Heat Shock Protein; immunohistochemistry; pyrethroid; TUNEL

Hrčak ID:

98682

URI

https://hrcak.srce.hr/98682

Publication date:

20.3.2013.

Article data in other languages: croatian

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