Original scientific paper
Isolation and Selection of Sulfur-oxidizing Bacteria for the Treatment of Sulfur-containing Hazardous Wastes
N.G. Rojas-Avelizapa
; aCentro de Investigación en Ciencia Aplicada y Tecnología Avanzada del IPN, Cerro Blanco 141, Col. Colinas del Cimatario 76090, Querétaro, Mexico
M. Gómez-Ramírez
; aCentro de Investigación en Ciencia Aplicada y Tecnología Avanzada del IPN, Cerro Blanco 141, Col. Colinas del Cimatario 76090, Querétaro, Mexico
R. Hernández-Gama
; aCentro de Investigación en Ciencia Aplicada y Tecnología Avanzada del IPN, Cerro Blanco 141, Col. Colinas del Cimatario 76090, Querétaro, Mexico
J. Aburto
; bInstituto Mexicano del Petróleo, Eje Central Lázaro Cárdenas 152, Col. San Bartolo Atepehuacan, 07730, México D. F., México
R. García de León
; bInstituto Mexicano del Petróleo, Eje Central Lázaro Cárdenas 152, Col. San Bartolo Atepehuacan, 07730, México D. F., México
Abstract
A total of 75 microorganisms were obtained from high-sulfur content environmental samples using different sulfur sources. Fifty-four of them had the ability to oxidize sulfur at 1% (w/v) in liquid culture, however only three of them AZCT-M125-5, AZCT- -M125-6, and AZCT-M125-7 were able to grow autotrophycally using elemental sulfurat concentrations higher than 1 % and up to 9 % (w/v) as energy source. They producemore than 300 mg sulfate/L. Also, these microbial cultures were able to produce sulfate
within pH 3 to 7. Analysis based on 16S rRNA gene sequences indicated that microbial cultures AZCT-M125-5 and AZCT-M125-6 were closely related to Acidithiobacillusthiooxidans while identification of AZCT-M125-7 was not possible. According to the results, these three microorganisms can be excellent candidates for the future development of alternative biotechnological processes for the treatment of hazardous wastes containing
sulfur.
Keywords
Sulfur-oxidizing; isolates; chemolithotrophic; acidophilic
Hrčak ID:
99444
URI
Publication date:
29.3.2013.
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