Original scientific paper

The Effect of Cycloheximide on Butyrylcholinesterase Activity In Vivo

Ana Lucić
Vlasta Bradamante
Božica Radić
Maja Peraica
Ana-Marija Domijan
Radovan Fuchs

Abstract

The paper describes the catalytic activity of butyrylcholinesterase (BuChE) measured in plasma, liver, white adipose tissue, heart, and brain of rats intraperitoneally administered a single non–lethal dose of cycloheximide (2.0 mg/kg body weight; CHM). The BuChE assay was performed on rats of both sexes either administered CHM or saline (controls), and killed 2, 3, 4, 5, 10 days later. A significant decrease of BuChE catalytic activity was observed in all tested tissues except plasma. In animals of both sexes, the lowest BuChE catalytic activity was found in the liver (2–6%), while it was higher in white adipose tissue, heart, and brain. However, the respective values remained significantly different from controls (33–67%, 49–62%, and 14–71% in males, and 24–82%, 72–86%, and 33–67% in females). Since there was no effect of CHM on BuChE catalytic activity in plasma, the data suggest that CHM inhibits the synthesis of BuChE rather than its active site.

Keywords

brain; heart; liver; plasma; protein synthesis; rat; white adipose tissue

Hrčak ID:

427

URI

https://hrcak.srce.hr/427

Publication date:

23.12.2002.

Article data in other languages: croatian

Visits: 1.391 *