Original scientific paper

Arginase status in cattle reproductive system.

Nematollah Razmi ; Department of Biochemistry, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Gholam Ali Jelodar ; Department of Biochemistry, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Saeed Nazifi ; Department of Biochemistry, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Abdorrrasoul Dehghani ; Department of Biochemistry, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

Abstract

Seven healthy, sexually adult cows were slaughtered and the whole reproductive system of all animals was immediately collected. Different tissues, including ovaries with corpora lutea, muscular and mucosal layers of uterine horn, uterine body, cervix, vagina and vestibula, were carefully separated. Arginase specific activity (ASA) was determined and compared by the modified paranitrophenylglyoxal (PNPG) method. Results of this study indicate that the highest arginase-specific activity (79.01±13.20 IU/mg of protein) is present in mucosal layer of vestibula, which did showed no significant difference with the mucosal layer of uterine horn (49.45±8.73) and muscular layer of vestibula (49.04±9.43) although there was a significant difference with the remaining parts of the reproductive system (P<0.05). The finding of this study also indicates that this enzyme is present at different levels in all parts of cattle reproductive system, which may be related to different rate of cell proliferation, differentiation or some other unknown physiological and biochemical activities of the enzyme in this system.

Keywords

arginase; reproductive system; cattle

Hrčak ID:

67032

URI

https://hrcak.srce.hr/67032

Publication date:

20.2.2005.

Article data in other languages: croatian

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