Original scientific paper

Effect of epidermal and insulin-like growth factor-1 on cumulus expansion, nuclear maturation and fertilization of buffalo cumulus oocyte complexes in simple serum free media DMEM and Ham’s F-10.

Dinesh Kumar ; Department of Animal Reproduction Gynaecology and Obstetrics, College of Veterinary andAnimal Science, Rajasthan Agricultural University, Bikaner (Raj.) India
Govind Narayan Purohit ; Department of Animal Reproduction Gynaecology and Obstetrics, College of Veterinary andAnimal Science, Rajasthan Agricultural University, Bikaner (Raj.) India

Abstract

The object of this study was to identify the possible effects of epidermal growth factor (EGF) and insulin-like growth factor 1 (IGF-1) on cumulus expansion, nuclear maturation and fertilization of buffalo cumulus oocyte complexes (COC’s) matured and fertilized in vitro in two serum free media Dulbecco’s modified Eagles medium (DMEM) and Ham’s F-10. Oocytes were matured in vitro for 24 h either in DMEM or Ham’s F-10 with no supplements (control) or in DMEM or Ham’s F-10 media supplemented with EGF, IGF-1 or with both EGF and IGF- 1. After 24 h of in vitro maturation, oocytes were inseminated with sperm prepared in BO medium supplemented with 10 μg ml-1 heparin and 0.5% BSA. In Experiment 1 the proportion of oocytes showing maximum cumulus expansion was significantly higher (P<0.01) in DMEM medium supplemented with EGF plus IGF-1 at a rate of 51.2% versus the control at a rate of 18.0%. In Ham’s F-10 media a significantly higher (P<0.01) proportion of oocytes showed maximum expansion when the medium was supplemented with EGF and IGF-1 at a rate of 52.9% versus 19.4% for the control. The same oocytes, when fixed and assessed for nuclear maturation, showed that a significantly higher (P<0.01) number of oocytes had reached M-II in both DMEM and Ham’s F-10 supplemented with EGF and IGF-1 at a rate of 78.0% and 83.5% versus a rate of 43.0% and 46.3% in their respective controls. In Experiment 2, 24 h after insemination a significantly higher (P<0.01) proportion of oocytes were fertilized in both DMEM and Ham’s F-10 supplemented with both EGF and IGF-1 at a rate of 45.2% and 48.6% versus a rate of 14.6% and 15.0% in their respective controls. These results lead to the following conclusions: (i) supplementation of both DMEM and Ham’s F-10 with both EGF and IGF-1 improves cumulus expansion, nuclear maturation and fertilization of buffalo cumulus oocyte complexes; (ii) both serum free media DMEM and Ham’s F-10 seem to have the same effect on cumulus expansion, nuclear maturation and fertilization of buffalo oocytes.

Keywords

buffalo; epidermal growth factor; insulin-like growth factor; oocyte

Hrčak ID:

67628

URI

https://hrcak.srce.hr/67628

Publication date:

21.2.2004.

Article data in other languages: croatian

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