Veterinary Archives, Vol. 74 No. 2, 2004.
Original scientific paper
Effect of paraquat intoxication and ambroxol treatment on hydrogen peroxide production and lipid peroxidation in selected organs of rat.
Anguel Anguelov
; Regional Veterinary Station, Plovdiv, Bulgaria
Maria Chichovska
; Medical University, Plovdiv, Bulgaria
Abstract
Experiments with the herbicide paraquat were carried out on Wistar rats. The concentration of hydrogen peroxide (H2O2) and various lipid peroxidation products (LPP), such as conjugated dienes (CD), lipid peroxides (LH), malonyl-dialdehyde (MDA) and Schiff bases in selected organs of the rat given a single intra-peritoneal dose of 35 mg/kg-1 paraquat was examined. The influence of a mucolytic and probably antioxidant drug, ambroxol, on paraquat-induced changes in the concentration of H2O2 and LPP was also examined. Paraquat increased the pulmonic, cardiac and hepatic concentration of H2O2, CD, LH and MDA approximately fourfold. Although the dose of paraquat was nearly twice the LD50 dose, it did not noticeably increase the concentration of these substances in the kidney. Ambroxol alleviated the increase of H2O2 in the liver but did not reduce the concentration of CD, LH and CD. The results indicate that ambroxol did not alleviate the increase the pulmonic concentration of H2O2, but protected the increase in the concentration of CD and MDA. Moreover, the drug administration alone induced lipid peroxidation in the liver. Ambroxol alone acts as a pro-oxidant.
Keywords
paraquat; ambroxol; hydrogen peroxide; lipid peroxidation
Hrčak ID:
67854
URI
Publication date:
22.4.2004.
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