Original scientific paper

Production, characterization and application of monoclonal antibodies against chicken IgY.

Gopal Dhinakar Raj ; Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, India
Bavani Latha ; Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, India
Meenakshi Sundaram Chandrasekhar ; Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, India
Venkatachalam Thiagarajan ; Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, India

Abstract

Monoclonal antibodies (Mabs) were produced against chicken IgY purified from egg yolks. Purity of the IgY was established using immunoelectrophoresis and reducing and non reducing polyacrylamide gel electrophoresis. Five stable hybrid clones were characterized and Mabs, all belonging to IgG1 isotype, were found to bind to the IgY heavy chain in Western blots. These clones did not cross-react with IgM in enzyme-linked immunosorbent assay (ELISA). One of the Mabs was used as a capture antibody in ELISA for estimation of IgY purified from egg yolk by different methods. Caprylic acid precipitation method gave the highest recovery of IgY and with high purity.

Keywords

chicken IgY; monoclonal antibodies; enzyme-linked immunosorbent assay; polyacrylamide gel electrophoresis; caprylic acid

Hrčak ID:

67879

URI

https://hrcak.srce.hr/67879

Publication date:

21.6.2004.

Article data in other languages: croatian

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