Original scientific paper

IN VITRO BULB PRODUCTION IN HIPPEASTRUM (HIPPEASTRUM HYBRIDUM)

J. Sutlana
N. Sutlana
M.N.A. Siddique
A.k.M.A. Islam
M.M. Hossain
T. Hossain

Abstract

An in vitro experiment was conducted to find out the optimum hormonal supplement and sucrose level for the bulb
production of Hippeastrum. Murashige and Skoog medium supplemented with different hormone concentrations of
BAP (0.0, 2.0, 4.0, 6.0 and 8.0 mg/L) and CCC (0.0, 125, 250 and 500 mg/L) and sucrose levels (30, 60, 80, 90 and
110 g/L) were used in this study. Sucrose level at 90 g/L produced the maximum average weight as well as the highest
regeneration percentage. The increasing rate of CCC increased the number and average weight of bulb. The maximum
bulb formation observed in media supplement with 6.0 mg/L BAP and 500 mg/L CCC fortified with 90 g/L sucrose.

Keywords

Hippeastrum (Hippeastrum hybridum); In vitro; bulb production; tissue culture; BAP; CCC; sucrose

Hrčak ID:

68173

URI

https://hrcak.srce.hr/68173

Publication date:

20.1.2011.

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