Original scientific paper

Histochemical distribution of digestive enzymes in hake, Merluccius merluccius L.

Zvonimir Kozarić ; Department of Anatomy, Histology and Embryology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Snježana Kužir ; Department of Anatomy, Histology and Embryology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Srebrenka Nejedli ; Department of Anatomy, Histology and Embryology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Zdravko Petrinec ; Department of Biology and Pathology of Fish and Bees, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Emil Srebočan ; Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia

Abstract

Histochemical localization of non-specific esterase, alkaline and acid phosphatase in the digestive tract of free-living Hake (Merluccius merluccius L. 1758) and their contribution to the digestive process were researched. Material originated from the Adriatic Sea (vicinity of the Island of Rab). Histochemical techniques were used for detection of enzymatic activity. Various enzymatic activities had been noticed, in correlation with particular parts of fish digestive tract. Strong esterase activity had been noticed in intestinal epithelial cells. Alkaline phosphatase has a wide distribution and localization in intestine segments, generally in lamina propria, but mostly in brush border of enterocytes. Activity of acid phosphatase has been associated with intestinal epithelial cells, and it is the only enzyme to be detected in gastric glands. Obtained data will have an influence on understanding the digestive processes of investigated fish.

Keywords

Merluccius merluccius; digestive tract; enzymes; histochemistry

Hrčak ID:

68178

URI

https://hrcak.srce.hr/68178

Publication date:

21.8.2004.

Article data in other languages: croatian

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