Original scientific paper

Histochemical investigations of NADPH- and NADH-tetrazolium reductase activity in the liver of selenium and copper treated carp (Cyprinus carpio L.)

Nada Kralj-Klobučar ; Department of Biology, Faculty of Science, University of Zagreb, Croatia
Margita Gorup ; Department of Biology, Faculty of Science, University of Zagreb, Croatia

Abstract

The effects of long-term intoxication with low concentrations of selenium and copper on NADPH- or NADH-tetrazolium reductase activity was examined. Carp were separately treated with selenium or copper in concentrations of 0.1 mg/L for 52 days. A non-treated group served as control. Activity of NADPH- and NADH-tetrazolium reductase was demonstrated in the liver tissue using histochemical methods according to Scarpelli et al. NADPH- and NADH-tetrazolium reductase activities were located in intracellular granules. NADH-tetrazolium reductase also showed diffuse distribution in the cytoplasm. Granules possessing the activity of both enzymes represented mitochondria. Results showed that selenium and copper in concentrations of 0.1 mg/L did not induce alterations in NADPH-TR activity over a 52-days period. Treatment with both elements induced oscillations of NADH-TR activity in granules, while copper also induced a significant incrase of enzyme activity in the cytoplasm. Prolonged intoxication (after 49 days) with both selenium and copper led to damage to liver tissue and to reduced activity of both enzymes.

Keywords

carp; liver; selenium; copper; histochemistry; NADPH- and NADH-tetrazolium reductase

Hrčak ID:

68595

URI

https://hrcak.srce.hr/68595

Publication date:

20.10.2004.

Article data in other languages: croatian

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