Original scientific paper

Serodiagnosis of nasal and visceral schistosomosis in cattle by counter current immuno electrophoresis.

Sreenivasiah Sumanth ; Centre of Advanced Studies, Department of Parasitology, University of Agricultural Sciences, Veterinary College, Hebbal, Bangalore, India
Placid Eugene D’Souza ; Centre of Advanced Studies, Department of Parasitology, University of Agricultural Sciences, Veterinary College, Hebbal, Bangalore, India
Mitta Srinivasa Jagannath ; Centre of Advanced Studies, Department of Parasitology, University of Agricultural Sciences, Veterinary College, Hebbal, Bangalore, India

Abstract

Counter current immunoelectrophoresis (CIEP) was evaluated for serodiagnosis of nasal and visceral schistosomosis in cattle. The whole worm antigens of Schistosoma nasale and S. spindale with protein content of 3.5mg/ml were prepared and the CIEP test was conducted. A clear cut positive band indicating positivity was observed in hyperimmune sera raised against both species. This technique was 98.62 and 99.50% sensitive in S. nasale and S. spindale infections in known positive cattle, respectively. CIEP could also detect infections in 85.36% and 92.70% of the cattle in which neither eggs nor worms could be recovered on post-mortem indicating previous exposure to infection.

Keywords

Schistosoma nasale; S. spindale; serodiagnosis; counter current immuno electrophoresis; cattle

Hrčak ID:

69135

URI

https://hrcak.srce.hr/69135

Publication date:

21.12.2004.

Article data in other languages: croatian

Visits: 1.302 *