Acta Pharmaceutica, Vol. 61 No. 3, 2011.
Original scientific paper
https://doi.org/10.2478/v10007-011-0027-1
Simultaneous estimation of ramipril, acetylsalicylic acid and atorvastatin calcium by chemometrics assisted UV-spectrophotometric determinations in capsules
A. S. KAMATCHI SANKAR
; Adhiparasakthi College of Pharmacy, Melmaruvathur-603 319, India
THANGARASU VETRICHELVAN
; Adhiparasakthi College of Pharmacy, Melmaruvathur-603 319, India
DEVASHYA VENKAPPAYA
; School of Biotechnology,(Shanmuga Arts, Science, Technology and Research Academy), SASTRA University, Thanjavur-613 402, India
Abstract
In the present work, three different spectrophotometric methods for simultaneous estimation of ramipril, aspirin and atorvastatin calcium in raw materials and in formulations are described. Overlapped data was quantitatively resolved by using chemometric methods, viz. inverse least squares (ILS), principal component regression (PCR) and partial least squares (PLS). Calibrations were constructed using the absorption data matrix corresponding to the concentration data matrix. The linearity range was found to be 1-5, 10-50 and 2-10 µg mL–1 for ramipril, aspirin and atorvastatin calcium, respectively. The absorbance matrix was obtained by measuring the zero-order absorbance in the wavelength range between 210 and 320 nm. A training set design of the concentration data corresponding to the ramipril, aspirin and atorvastatin calcium mixtures was organized statistically to maximize the information content from the spectra and to minimize the error of multivariate calibrations. By applying the respective algorithms for PLS 1, PCR and ILS to the measured spectra of the calibration set, a suitable model was obtained. This model was selected on the basis of RMSECV and RMSEP values. The same was applied to the prediction set and capsule formulation. Mean recoveries of the commercial formulation set together with other figures of merit (calibration sensitivity, selectivity, limit of detection, limit of quantification and analytical sensitivity) were estimated. Validity of the proposed approaches was successfully assessed for analyses of drugs in the various prepared physical mixtures and formulations.
Keywords
figures of merit; multivariate calibration; ramipril; acetylsalicylic acid; atorvastatin calcium
Hrčak ID:
69436
URI
Publication date:
1.9.2011.
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