Original scientific paper

Antimicrobial resistance, phenotypic characteristics and phage types of B. abortus strains isolated from cattle and water buffalo (Bubalus bubalis) in Trinidad.

Abiodun A. Adesiyun ; School of Veterinary Medicine, Faculty of Medical Sciences, University of the West Indies, St. Augustine, Trinidad and Tobago
Kadian Baird ; School of Veterinary Medicine, Faculty of Medical Sciences, University of the West Indies, St. Augustine, Trinidad and Tobago
Alva Stewart-Johnson ; School of Veterinary Medicine, Faculty of Medical Sciences, University of the West Indies, St. Augustine, Trinidad and Tobago

Abstract

Strains of Brucella abortus isolated from cattle and domestic water buffalo (Bubalus bubalis) in Trinidad and Tobago were characterized as to their phenotypic features, phage types and resistance to antimicrobial agents using standard methods. A total of 86 isolates were recovered from the lymph nodes of 14 apparently healthy seropositive cattle and 17 water buffalo, skin lesions of 9 water buffalo and aborted tissues of 16 water buffalo. In addition 2 vaccine strains, B. abortus strains 19 (S19) and RB51 (SRB51) were tested. All (100.0%) strains of B. abortus tested grew in the presence of penicillin G, i-erythritol and basic fuchsin but none (0.0%) grew in the presence of thionin blue. All 88 (100.0%) strains of B. abortus were susceptible to bacteriophages TB and BK2 but 84 (95.5%) were lysed by bacteriophage Wb. Of the 8 antimicrobial agents tested by the disc diffusion method, all 42 (100.0%) cattle and water buffalo carried resistant isolates and all 88 (100.0%) isolates of B. abortus exhibited resistance to one or more of the antimicrobial agents. All sources considered, resistance was high to azithromycin (100.0%), sulphamethoxazole/trimethoprim (98.9%) and moxifl oxacin (80.7%) and low to streptomycin (5.7%), tetracycline (1.1%) and doxycycline (1.1%). The differences in prevalence of resistance of B. abortus isolates to antimicrobial agents were statistically significant (P<0.05; χ2) but isolates from cattle and water buffalo had similar prevalence of resistance (P>0.05; χ2). Resistance to antimicrobial agents used in the treatment of human brucellosis poses a public health hazard, but most of the strains had similar phenotypic characteristics and bacteriophage susceptibility patterns.

Keywords

phage types; antibiotic resistance; Brucella abortus; cattle; water buffalo

Hrčak ID:

69494

URI

https://hrcak.srce.hr/69494

Publication date:

20.6.2011.

Article data in other languages: croatian

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