Agriculture, Vol. 17 No. 1, 2011.
Original scientific paper
DIFFERENTIATION OF CROATIAN BARLEY VARIETIES BY GRADIENT GEL SDS-PAGE AND ISOELECTRIC FOCUSING OF DRY GRAINS AND GREEN MALT HORDEINS
Ivica Strelec
orcid.org/0000-0003-3999-8499
; J.J. Strossmayer University of Osijek, Faculty of Food Technology - Franje Kuhača 20, HR-31000 Osijek, Croatia
Elzabeta Has-Schön
; Department of Biology, Gajev trg 6, HR-31000 Osijek, Croatia
Ljubinka Vitale
; Ruđer Bošković Institute, Bijenička cesta 54, HR-10000 Zagreb
Abstract
Applicability of polyacrylamide gel electrophoresis and isoelectric focusing of hordeins for discrimination of six two-rowed winter barley varieties (Angora, Sladoran, Rodnik, Rex, Martin and Barun) has been investigated. Hordeins extracted from dry grains and green malt of barley varieties were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis in gradient gel of 8-18% T, and by isoelectric focusing in pH gradient of 3.5-9.5 and 5.5-8.5, respectively. In all separation experiments better resolution of proteins was achieved with dry grain extracts, than with malt extracts. Angora, Sladoran and Martin variety could be distinguished from other varieties by differences in hordein patterns obtained by gradient gel SDS-PAGE (8-18% T), and Angora, Sladoran, Martin and Rodnik by isoelectric focusing in pH gradient 5.5-8.5.
Keywords
barley variety discrimination; gradient gel SDS-PAGE; hordeins; isoelectric focusing
Hrčak ID:
69540
URI
Publication date:
21.6.2011.
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