Biochemia Medica, Vol. 21 No. 2, 2011.
Original scientific paper
Lipemia interferences in routine clinical biochemical tests
Pilar Calmarza
; Laboratory of Clinical Biochemistry, University Hospital Miguel Servet, Zaragoza, Spain
Jose Cordero
; Research Unit, Hospital General Yagüe, Burgos, Spain
Abstract
Introduction: Lipemic specimens are a common and frequent, but yet unresolved problem in clinical chemistry, and may produce significant interferences in the analytical results of different biochemical parameters.
Material and methods: The aim of this study was to examine the effect of lipid removal using ultra-centrifugation of lipemic samples, on some routine biochemistry parameters. Among all the samples obtained daily in our laboratory, the ones which were visibly muddy were selected and underwent to a process of ultracentrifugation, being determined a variety of biochemical tests before and after ultra-centrifugation. A total of 110 samples were studied.
Results: We found significant differences in all the parameters studied except for total bilirubin, glu-cose, gamma-glutamyl transferase (GGT) and aspartate aminotransferase (AST). The greatest diffe-rences in the parameters analyzed were found in the concentration of alanine aminotransferase (ALT) (7.36%) and the smallest ones in the concentration of glucose (0.014%). Clinically significant interfe-rences were found for phosphorus, creatinine, total protein and calcium.
Conclusion: Lipemia causes clinically significant interferences for phosphorus, creatinine, total prote-in and calcium measurement and those interferences could be effectively removed by ultracentrifuga-tion.
Keywords
lipemia; ultracentrifugation; biochemical tests; interferences
Hrčak ID:
69663
URI
Publication date:
15.6.2011.
Visits: 5.512 *