Original scientific paper

Lipemia interferences in routine clinical biochemical tests

Pilar Calmarza ; Laboratory of Clinical Biochemistry, University Hospital Miguel Servet, Zaragoza, Spain
Jose Cordero ; Research Unit, Hospital General Yagüe, Burgos, Spain

Abstract

Introduction: Lipemic specimens are a common and frequent, but yet unresolved problem in clinical chemistry, and may produce significant interferences in the analytical results of different biochemical parameters.
Material and methods: The aim of this study was to examine the effect of lipid removal using ultra-centrifugation of lipemic samples, on some routine biochemistry parameters. Among all the samples obtained daily in our laboratory, the ones which were visibly muddy were selected and underwent to a process of ultracentrifugation, being determined a variety of biochemical tests before and after ultra-centrifugation. A total of 110 samples were studied.
Results: We found significant differences in all the parameters studied except for total bilirubin, glu-cose, gamma-glutamyl transferase (GGT) and aspartate aminotransferase (AST). The greatest diffe-rences in the parameters analyzed were found in the concentration of alanine aminotransferase (ALT) (7.36%) and the smallest ones in the concentration of glucose (0.014%). Clinically significant interfe-rences were found for phosphorus, creatinine, total protein and calcium.
Conclusion: Lipemia causes clinically significant interferences for phosphorus, creatinine, total prote-in and calcium measurement and those interferences could be effectively removed by ultracentrifuga-tion.

Keywords

lipemia; ultracentrifugation; biochemical tests; interferences

Hrčak ID:

69663

URI

https://hrcak.srce.hr/69663

Publication date:

15.6.2011.

Visits: 5.561 *