Original scientific paper

Trypanosoma evansi possesses a gene similar to putative Trypanosoma brucei rhodesiense oligosaccharyl transferase clone I.

Waren N. Baticados ; Department of Veterinary Paraclinical Sciences, College of Veterinary Medicine, University of the Philippines Los Baños, Laguna, Philippines
Noboru Inoue ; National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan
Chihiro Sugimoto ; Research Center for Zoonosis Control, Hokkaido University, Kita-Ku, Sapporo, Hokkaido, Japan
Hideyuki Nagasawa ; National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan
Abigail M. Baticados ; Department of Veterinary Paraclinical Sciences, College of Veterinary Medicine, University of the Philippines Los Baños, Laguna, Philippines

Abstract

Recent data has shown that there are strong indications that the putative oligosaccharyl transferase genes from Trypanosoma brucei rhodesiense were conserved within the family Trypanosomatidae. Based on these findings, the study endeavored to determine if Trypanosoma evansi also possessed putative oligosaccharyl transferase (OST) clone I previously documented in Trypanosoma brucei rhodesiense. Using the DNA hybridization method (Southern blot analyses), genomic DNAs of Trypanosoma brucei rhodesiense and Trypanosoma evansi were processed using the same set of restriction enzymes and subsequently hybridized by the same set of DNA probes designed from the reported nucleotide sequence of Trypanosoma brucei rhodesiense putative oligosaccharyl transferase clone I. The results exhibited that Trypanosoma evansi also contains a gene similar to the reported Trypanosoma brucei rhodesiense putative OST gene clone I, as shown by the successful hybridization of the DNA probes to their complementary nucleotide sequences in the genome of the Trypanosoma evansi species. In addition, the data also showed that Trypanosoma brucei rhodesiense and Trypanosoma evansi genomes shared some common restriction sites and loci within the genome of each individual parasite species.

Keywords

Trypanosoma evansi; oligosaccharyl transferase; N-glycosylation; DNA hybridization

Hrčak ID:

70930

URI

https://hrcak.srce.hr/70930

Publication date:

20.7.2011.

Article data in other languages: croatian

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