Original scientific paper

https://doi.org/10.5562/cca1748

Changes in Erythrocyte Transmethylation Ability are Predictive Factors for Tumor Prognosis in Prostate Cancer

Anna Blázovics ; Department of Pharmacognosy, Semmelweis University, H-1085 Budapest, Üllői Rd. 2, Hungary; II. Department of Medicine, Semmelweis University, H-1088 Budapest, Szentkirályi Str.46, Hungary
Péter Nyirády ; Department of Urology, Semmelweis University, 1082 Budapest, Üllői Rd. 78/b, Hungary
Gabriella Bekő ; Central Laboratory Pest, Semmelweis University, 1083 Budapest, Korányi S. Str. 2/a, Hungary
Edit Székely ; II. Department of Medicine, Semmelweis University, H-1088 Budapest, Szentkirályi Str.46, Hungary
Ágnes Szilvás ; Saint John Hospital, H-1125 Budapest, Diós ditch 1-3, Hungary
Eszter Kovács-Nagy ; Department of Genetic and Plant Breeding, Faculty of Horticultural Science, Corvinus University of Budapest, H-1118 Villányi Rd. 29-43, Budapest, Hungary
András Horváth ; Department of Urology, Semmelweis University, 1082 Budapest, Üllői Rd. 78/b, Hungary
Miklós Szűcs ; Department of Urology, Semmelweis University, 1082 Budapest, Üllői Rd. 78/b, Hungary
Imre Romics ; Department of Urology, Semmelweis University, 1082 Budapest, Üllői Rd. 78/b, Hungary
Éva Sárdi ; Department of Genetic and Plant Breeding, Faculty of Horticultural Science, Corvinus University of Budapest, H-1118 Villányi Rd. 29-43, Budapest, Hungary

Abstract

Endogenous transmethylation processes proceed via formaldehyde. Endogenous formaldehyde can
be found in special bounded forms in animal tissues. The aim was to find non-invasive methods to help establish
an early diagnosis of prostate cancer processes, and to search for a reliable examination repertoire to predict
metastasis based on genomic hypomethylation that can be found in tumors. Fifty-eight age-matched patients
suffering from prostate cancer and 26 healthy volunteers were enrolled in the study. Biochip technique
was applied to quantify serum interleukins and growth factors. OPCL method served to determine mobilized
formaldehyde, and fluorometric methods were applied to measure Zn- and free protoporphyrin concentrations
in erythrocytes. Spectrophotometric measurements were carried out to evaluate redox homeostasis in
plasma. There were no significant differences in most measured laboratory and immune parameters at different
early stages of prostate cancer; at the same time, erythrocyte parameters, especially immobilized formaldehyde
determination, were promising. The authors assume that hypomethylation detection in erythrocytes
might become a predictive factor in the diagnostic panel in the near future. (doi: 10.5562/cca1748)

Keywords

hypomethylation; formaldehyde; protoporphyrin; prostate cancer; redox homeostasis

Hrčak ID:

71975

URI

https://hrcak.srce.hr/71975

Publication date:

3.10.2011.

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