Original scientific paper

PCR detection of Sudanese isolates of bluetongue virus serogroup.

Mohammed Abdalla ; Department of Medicine, Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Khartoum, Sudan
Siham Suliman ; Department of Medicine, Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Khartoum, Sudan
Abdelrahim Karrar ; Department of Medicine, Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Khartoum, Sudan
Salah Idris ; Central Veterinary Research Laboratories, Soba, Khartoum, Sudan
Imadeldin Aradaib ; Department of Medicine, Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Khartoum, Sudan

Abstract

The potential use of the recently reported reverse transcriptase (RT) polymerase chain reaction (RT-PCR)-based assay for detection of North American isolates of bluetongue virus (BTV) ribonucleic acid in cell culture was evaluated for detection of Sudanese isolates of BTV. Two pairs of oligoribonucleotide primers, selected from non-structural protein 1 (NS1) gene of BTV-17, were used for RT-PCR amplification. The BTV RT-PCR produced an 826 base pair (bp) amplification product. RNA from Sudanese BTV serotypes 4 and 16, propagated in cell cultures, were detected by this RT-PCR-based assay. Amplification product was not detected when the nested BTV RT-PCR based assay was applied to RNA from closely related Sudanese Orbivirus, epizootic hemorrhagic disease virus (EHDV) serotype 4 and total nucleic acid extracts from uninfected Vero cells. The results of this study indicated that our previously described BTV RT-PCR assay could be used for detection of the Sudanese BTV isolates and possibly other serotypes of BTV serogroup from different continents.

Keywords

orbiviruses; bluetongue virus; reverse transcriptase-polymerase chain reaction; Sudan

Hrčak ID:

74727

URI

https://hrcak.srce.hr/74727

Publication date:

22.6.2003.

Article data in other languages: croatian

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