Original scientific paper

https://doi.org/10.5562/cca1762

Enantioseparation of Dansylated Amino Acids by Ligand-exchange Capillary Electrophoresis Using L-phenylalaninamide, L-lysine or L-threonine as Chiral Selector

Stefan Mohr ; Department of Pharmaceutical Chemistry, Institute of Pharmaceutical Sciences, Karl-Franzens-University Graz, Universitätsplatz 1, A-8010 Graz, Austria
Johannes S. Hägele ; Department of Pharmaceutical Chemistry, Institute of Pharmaceutical Sciences, Karl-Franzens-University Graz, Universitätsplatz 1, A-8010 Graz, Austria
Martin G. Schmid ; Department of Pharmaceutical Chemistry, Institute of Pharmaceutical Sciences, Karl-Franzens-University Graz, Universitätsplatz 1, A-8010 Graz, Austria

Abstract

In recent years enantioseparation of both active pharmaceutical ingredients and bio molecules
such as amino acids became more and more necessary because in most cases the two stereo forms exhibit
different pharmacological effects. This article deals with the chiral separation of dansylated amino acids
by ligand-exchange capillary electrophoresis using L-phenylalaninamide, L-lysine and L-threonine as chiral
selectors. Experiments with different central metal ions such as Cu(II), Co(II), Cd(II), Ni(II) and Zn(II)
were carried out. Optimal conditions were found out by studying the effect of the pH and the selector molarity
on the chiral resolution. Best separation was obtained for the Cu(II)/L-lysine complex, showing a
chiral resolution up to 17 for Dns-DL-Met. (doi: 10.5562/cca1762)

Keywords

LECE; dansylated amino acids; CZE; chiral separation; ligand-exchange; capillary electrophoresis

Hrčak ID:

74770

URI

https://hrcak.srce.hr/74770

Publication date:

13.11.2011.

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