Original scientific paper

PCR-based randomly amplified polymorphic DNA (RAPD) fingerprinting for detection of genetic diversity among Sudanese isolates of Haemophilus somnus.

Kamaledin Eltoum ; Institute for Promotion of Animal Export, University of Khartoum, Khartoum, Sudan
Imadeldin Aradaib ; Molecular Biology Laboratory, Department of Medicine, Pharmacology and Toxicology, Faculty of Veterinary Medicine, Khartoum North, Sudan
Suliman El-Sanousi ; Institute for Promotion of Animal Export, University of Khartoum, Khartoum, Sudan

Abstract

Haemophilus somnus is a pathogen frequently associated with infectious thromboembolic meningoencephalitis in cattle. Five strains of H. somnus were isolated from clinically infected cattle. In the Sudan the organism is associated with a clinical syndrome characterized by nervous manifestations. The strains were found to be morphologically and serologically different as determined by cultural and biochemical characteristics. In the present study, a polymerase chain reaction (PCR)- based randomly amplified polymorphic DNA (RAPD) method was used to amplify H. somnus DNA using arbitrary oligonucleotide primers. The method was able to detect the heterogeneity of amplified DNA from the strains of H. somnus. The scientific data presented in this study suggests that RAPDPCR could be used as a valuable tool in the study of the the molecular epidemiology of H. somnus isolated from clinical samples of naturally infected cattle.

Keywords

Haemophilus somnus; randomly amplified polymorphic DNA (RAPD); PCR; Sudan

Hrčak ID:

76477

URI

https://hrcak.srce.hr/76477

Publication date:

21.12.2003.

Article data in other languages: croatian

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