Original scientific paper

Investigation of ABCA1 C69T polymorphism in patients with type 2 diabetes mellitus

H. Arzu Ergen orcid.org/0000-0001-5736-8453 ; Istanbul University, The Institute of Experimental Medicine, Department of Molecular Medicine, Capa-Istanbul,Turkey
Ümit Zeybek ; Istanbul University, The Institute of Experimental Medicine, Department of Molecular Medicine, Capa-Istanbul,Turkey
Özlem Gök ; Istanbul University, The Institute of Experimental Medicine, Department of Molecular Medicine, Capa-Istanbul,Turkey
Z. Eremis Karaali ; Department of Internal Medicine, Haseki Training and Research Hospital, Istanbul, Turkey

Abstract

Introduction: Non insulin dependent diabetes mellitus is the most common type of diabetes. Genetic factors, lipid profiles, hypertension are potential risk factors for diabetes mellitus. Adenosine binding cassette transporter proteins 1 (ABCA1) plays a role in cholesterol metabolism, especially high density lipoprotein (HDL-cholesterol). There are multiple mechanisms by which HDL-cholesterol can be atheroprotective, it is clear that the relative activity of ABCA1 plays a major role. We aimed to in-vestigate association of ABCA1 C69T gene polymorphism with lipid levels in Turkish type 2 diabetic patients.
Materials and methods: After isolation of DNA by ethanol precipitation we determined ABCA1 gene polymorphism by using polimerase chain reaction - restriction fragment lenght polymorphism (PCR-RFLP) method in 107 type 2 diabetic patients and 50 healthy controls.
Results: We have observed that the frequency of TT genotype is significantly higher in healthy con-trols compared to patients (14% vs. 3%; P = 0.008). Also frequency of T allele was higher in controls than in patients (34% vs. 21%; P = 0.020; OR (95% CI) = 0.52 (0.30-0.88)). There was no association of lipid levels and ABCA1 C69T polymorphism subgroups.
Conclusion: We have found significantly higher frequency of both T allele and genotype in control group when compared to patients that made us think that T allele may be a protective factor against diabetes mellitus. But, we could not find a relationship between genotypes and lipid concentrations in our two groups. Larger studies will help us to understand the relationship between ABCA1 C69T genotype and lipid parameters in diabetes mellitus.

Keywords

diabetes mellitus; lipoproteins; high density lipoprotein; dyslipidemia; polymorphism, ge-netic

Hrčak ID:

77405

URI

https://hrcak.srce.hr/77405

Publication date:

15.2.2012.

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