Original scientific paper

A simple enzyme-linked immunosorbent assay for the detection of Aeromonas spp.

Neelam Sachan ; FAO/WHO Collaborating Centre for Research and Training in Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar, India
Rajesh Kumar Agarwal ; FAO/WHO Collaborating Centre for Research and Training in Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar, India

Abstract

An indirect enzyme-linked immunosorbent assay employing antisera against somatic antigen and outer membrane protein of a rough Aeromonas strain was developed for the detection of Aeromonas spp. The test detected Aeromonas irrespective of its species or serotype. The optimum detection level was found to be 106/ml. The cross-reactions were observed only with Vibrio cholerae (O39) among the 15 different organisms belonging to Enterobacteriaceae, Vibrionaceae and a few Gram-positive pathogens tested. Preliminary trials on food samples revealed the test to be efficient in detecting Aeromonas spp.

Keywords

Aeromonas spp.; ELISA; outer membrane protein; food

Hrčak ID:

81605

URI

https://hrcak.srce.hr/81605

Publication date:

18.12.2002.

Article data in other languages: croatian

Visits: 1.035 *