Original scientific paper

Stability studies of common biochemical analytes in serum separator tubes with or without gel barrier subjected to various storage conditions

Serap Cuhadar ; Ataturk Training and Research Hospital, Department of Biochemistry and Clinical Biochemistry, Izmir, Turkey
Ayşenur Atay ; Ataturk Training and Research Hospital, Department of Biochemistry and Clinical Biochemistry, Izmir, Turkey
Mehmet Koseoglu ; Ataturk Training and Research Hospital, Department of Biochemistry and Clinical Biochemistry, Izmir, Turkey
Ahmet Dirican ; Ataturk Training and Research Hospital, Department of Oncology, Izmir,Turkey
Aysel Hur ; Ataturk Training and Research Hospital, Department of Biochemistry and Clinical Biochemistry, Izmir, Turkey

Abstract

Introduction: The collected and shipped blood samples are exposed to a various extra-analytical factors prior to analysis. The aim of the study was to determine the stability of analytes in serum gel tubes and plain tubes exposed to a range of storage temperatures and times after centrifugation.
Materials and methods: Fifteen healthy volunteers were recruited and venous blood was collected into four tubes, two with and two without gel separator. Analyzing the baseline samples in 30 min, all were stored at 4ºC or 24ºC for 6, 12, 18, 24, 30, 36, 48 and 72 hours and 1 week. Sixteen biochemi-cal anaytes were measured on each sample. Variations remained under the desirable bias conside-red as clinically insignificant.
Results: On day three, most analytes remained stable including albumin, protein, creatinine, choles-terol, triglycerides, gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), alanine aminot-ransferase (ALT), creatine kinase (CK), lactate dehydrogenase (LD) regardless of tube types. Gluco-se concentration decreased markedly (P = 0.001) beginning from the first hours of storage in plain serum. The stability maximized for the analytes including glucose, total bilirubin, urea nitrogen (BUN), uric acid stored at 4 ºC in gel tubes. Aspartate aminotransferase (AST) activity increased significantly (P = 0.002) up to 48-h, however bias was not significant clinically. High density lipoprotein (HDL) con-centration was stable in gel tubes at 24 ºC, in plain tubes at 4 ºC stored up to 36-h.
Conclusion: Serum gel or non-gel tubes might be used interchangeably for 11 analytes chilled or at 24 ºC, whereas some restrictions must be applied for glucose, AST, BUN, HDL, and uric acid.

Keywords

blood specimen collection; enzyme stability; serum; storage; temperature

Hrčak ID:

83225

URI

https://hrcak.srce.hr/83225

Publication date:

15.6.2012.

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