Original scientific paper

Bovine tuberculosis in Bosnia and Herzegovina caused by Mycobacterium caprae.

Hajrudin Beširović orcid.org/0000-0002-5673-0753 ; University of Sarajevo, Veterinary Faculty, Department of Pathology, Sarajevo, Bosnia and Herzegovina
Amer Alić ; University of Sarajevo, Veterinary Faculty, Department of Pathology, Sarajevo, Bosnia and Herzegovina
Silvio Špičić ; Croatian Veterinary Institute, Department of Bacteriology and Parasitology, Laboratory for Bacterial Zoonosis and Molecular Diagnosis of Bacterial Diseases, Zagreb, Croatia
Željko Cvetnić ; Croatian Veterinary Institute, Department of Bacteriology and Parasitology, Laboratory for Bacterial Zoonosis and Molecular Diagnosis of Bacterial Diseases, Zagreb, Croatia
Senad Prašović ; University of Sarajevo, Veterinary Faculty, Department of Pathology, Sarajevo, Bosnia and Herzegovina
Lejla Velić ; University of Sarajevo, Veterinary Faculty, Department of Microbiology with Immunology and Infectious Diseases, Sarajevo, Bosnia and Herzegovina

Abstract

Bovine tuberculosis is a chronic disease of a zoonotic character that occurs in cattle and a wide range of domestic and wild animals. In this paper, bovine tuberculosis caused by M. caprae in six small household herds in Bosnia and Herzegovina was described. For the identification of the disease and determination of the causative agent comparative tuberculin skin test (TST), pathomorphology, microbiology and molecular methods were applied. Twenty-eight cows from five households from the Livno region (25/28 animals) and one household from the Vitez region (3/28 animals) were euthanized after being found positive by the comparative TST test. Eleven animals from five households from the Livno region (9/11 animals) and one household from the Vitez region (2/11 animals) were subject to field necropsy, and lesions consistent with tuberculosis were observed on the lymph nodes of the thoracic cavity and lungs in all carcasses. Histopathologic examination by hematoxylin and eosin staining confirmed the presence of specific granulomatous lesions, while Ziehl-Neelsen staining demonstrated the presence of very few acid fast bacteria. Mediastinal lymph nodes from seven necropsied animals (two animals from the Vitez region and five animals from five flocks from the Livno region) were submitted for bacteriology. Acid fast bacteria from five out of the seven submitted samples from one animal from the Vitez region and four animals from four households from the Livno region were isolated. All isolates were identified as M. caprae. By MIRU typing we found two different M. caprae genotypes, unique to Bosnia and Herzegovina. Our findings represent the first evidence of bovine tuberculosis caused by M. caprae in Bosnia and Herzegovina.

Keywords

cattle; tuberculosis; Mycobacterium caprae; Bosnia and Herzegovina; epidemiology

Hrčak ID:

84070

URI

https://hrcak.srce.hr/84070

Publication date:

4.7.2012.

Article data in other languages: croatian

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