Original scientific paper
https://doi.org/10.2478/10004-1254-63-2012-2227
Identification of Fusarium Species Isolated from Stored Apple Fruit in Croatia
Zdravka Sever
; Department of Plant Pathology, Faculty of Agriculture, University of Zagreb, Zagreb, Croatia
Dario Ivić
; Institute for Plant Protection, Croatian Centre for Agriculture, Food and Rural Affairs, Zagreb, Croatia
Tomislav Kos
; Department for Agricultural Zoology, Faculty of Agriculture Zagreb, University of Zagreb, Zagreb, Croatia
Tihomir Miličević
; University of Zagreb3 Zagreb, Croatia
Abstract
Several species of the genus Fusarium can cause apple fruit to rot while stored. Since Fusarium taxonomy is very complex and has constantly been revised and updated over the last years, the aim of this study was to identify Fusarium species from rotten apples, based on combined morphological characteristics and molecular data. We identified 32 Fusarium isolates from rotten apple fruit of cultivars Golden Delicious, Jonagold, Idared, and Pink Lady, stored in Ultra Low Oxygen (ULO) conditions. Fusarium rot was detected in 9.4 % to 33.2 % of naturally infected apples, depending on the cultivar. The symptoms were similar in all four cultivars: a soft circular brown necrosis of different extent, with or without visible sporulation. Fusarium species were identifi ed by the morphology of cultures grown on potato-dextrose agar (PDA) and carnation leaf agar (CLA). Twenty one isolates were identified as Fusarium avenaceum and confi rmed as such with polymerase chain reaction (PCR) using specific primer pair FA-ITSF and FA-ITSR. F. pseudograminearum, F. semitectum, F. crookwellense, and F. compactum were identifi ed by morphological characteristics. F. avenaceum can produce several mycotoxins and its dominance in Fusarium rot points to the risk of mycotoxin contamination of apple fruit juices and other products for human consumption. Pathogenicity tests showed typical symptoms of Fusarium rot in most of the inoculated wounded apple fruits. In this respect Fusarium avenaceum, as the dominant cause of Fusarium rot in stored apple fruits is a typical wound parasite.
Keywords
F. avenaceum; Fusarium rot; morphology; mycotoxins; pathogenicity; PCR
Hrčak ID:
93649
URI
Publication date:
14.12.2012.
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