Original scientific paper

Antimicrobial activity of Lactobacillus acidophilus

Jagoda Šušković orcid.org/0000-0003-1711-316X ; Prehrambeno-biotehnološki fakultet, Pierottijeva 6, 10000 Zagreb
Maša Krobot
Milena Mehak
S. Matošić

Abstract

Lactobacillus acidophilus is often mentioned as beneficial dietary adjunct. This organism possesses characteristics which permit its survival and growth in the intestinal tract. L. acidophilus has received most attention in being inhibitory toward the commonly known foodborne pathogens. The effect of different growth media on antimicrobial activity of L. acidophilus was examined in this paper. MRS and APT media with and without chalk (to bind accumulated lactic acid) were used for the growth of L. acidophilus. Culture broth, cell free supernatant and dialysate from L. acidophilus were examined for antimicrobial activity against the enteropathogenic microorganisms (Staphylococcus aureus 3048, Staph, aureus K-144, Salmonella mumum Escherichia coli) and the spore-forming bacteria Bacillus subtilis and Bacillus cereus. The best growth and the highest antimicrobial activity were achieved in MRS medium, after 48 hours of cultivation. The most sensitive microorganisms, have been Salmonella mumum, Escherichia coli and Bacillus subtilis.

Keywords

antibacterial activity of Lactobacillus acidophilus; L. acidophilus as dietary adjunct; bacteriocins; antibacterial substances

Hrčak ID:

95370

URI

https://hrcak.srce.hr/95370

Publication date:

10.4.1993.

Article data in other languages: croatian

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