Veterinary Archives, Vol. 83 No. 1, 2013.
Original scientific paper
Genetic characterization of turkey astroviruses identified in Croatian poultry fl ocks.
Marina Biđin
; Department of Poultry Diseases with Clinic, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Darko Majnarić
; Veterinary Department Križevci, Croatian Veterinary Institute, Križevci, Croatia
Zdenko Biđin
; Department of Poultry Diseases with Clinic, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Ivana Lojkić
; Department of Virology, Croatian Veterinary Institute, Zagreb, Croatia
Abstract
The identification and genetic characterization is described of turkey astroviruses (TAstV) detected by polymerase chain reaction (PCR) in Croatian turkey and chicken flocks. Multiple organ samples were collected from 16 turkey and 7 chicken flocks and tested by specific primers for the presence of turkey astrovirus-1 (TAstV-1) and turkey astrovirus-2 (TAstV-2). TAstV-1 was detected in 6 turkey flocks, and TAstV-2 in 4 turkey and 2 chicken flocks, which represents the first finding of turkey-origin astrovirus in chicken hosts. Amplified polymerase gene fragments were sequenced and analysed. Phylogenetic analysis was based on the alignments of 82 amino acids deduced from 250 nucleotide (nt) ORF 1b sequences from a total of 30 TAstV isolates. Molecular and phylogenetic analysis of astrovirus sequences revealed the existence of one TAstV-2 and two TAstV-1 genotypes detected in commercial poultry flocks in Croatia. Within each cluster of astroviruses, the polymerase gene proved to be highly conserved.
Keywords
turkey astrovirus-1; turkey astrovirus-2; genetic characterization; phylogenetic analysis
Hrčak ID:
97125
URI
Publication date:
11.2.2013.
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