Original scientific paper

Proteolytic Activity of Trichomonas tenax from Dentobacterial Plaque and Root Canal Contens

Sanja Šegović ; Zavod za bolesti zubi Stomatološki fakultet Gundulićeva 5, Zagreb, Hrvatska
Dunja Buntak-Kobler ; Zavod za bolesti zubi Stomatološki fakultet Gundulićeva 5, Zagreb, Hrvatska
Albert Marinculić ; Odjel za parazitologiju Veterinarski fakultet, Heinzelova 55, Zagreb, Hrvatska
Jasminka Granić ; Laboratorij za kliničku parazitologiju Klinička bolnica za infektivne bolesti “Dr. Fran Mihaljević”, Rockfellerova 3, Zagreb, Hrvatska
Dora Najžar-Fleger ; Zavod za bolesti zubi Stomatološki fakultet Gundulićeva 5, Zagreb, Hrvatska
Emir Duraković ; Odjel za parazitologiju Veterinarski fakultet, Heinzelova 55, Zagreb, Hrvatska

Abstract

The purpose of this study was to examine proteolytic activity of Trichomonas tenax collected directly from dentobacterial plaque and root canal contents o f gangrenous teeth or teeth with periapical processes. Both electrophoretic method involving polyacrylamide gels and electrophoretic method involving gelatin - containing polyacrylamide gels, have been used to analyse Trichomonas tenax proteolytic activity. The most obvious and fastest activities were obtained when gels were incubated in pH 4.6, followed by the results of incubating in pH 5.6, while in pH 2.8 activity was less but still obvious. Proteolytic activities were the most effective in the area of protein MW 36 kDa. The different activity of enzymes depending on the pH o f incubated media indicate the presence of different endopeptidases in cell lysates of protozoon Trichomonas tenax from dentobacterial plaque and root canal contents.

Keywords

Trichomonas tenax; proteolytic activity; dentobacterial plaque; root canal

Hrčak ID:

98250

URI

https://hrcak.srce.hr/98250

Publication date:

15.12.1998.

Article data in other languages: croatian

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