Original scientific paper

Cytotoxicity and Genotoxicity of Some Dental Alloys

Vlado Carek ; School of Dental Medicine, University of Zagreb, Zagreb, Croatia
Vjekoslav Jerolimov ; School of Dental Medicine, University of Zagreb, Zagreb, Croatia
Ružica Rozgaj ; Institut za medicinska istraživanja i medicinu rada, Zagreb, Hrvatska

Abstract

Cytotoxicity and genotoxicity of some alloys widely used in dentistry were studied for direct comparison. The study included cobalt-chromium and gold-platinum alloys, and samarium-cobalt magnets. Cylindrical preparations were sized 3.0 x 6.85 mm and cast according to manufacturers ’ instructions. In in vitro studies, a Saccharomyces cerevisiae yeast was used, whereas in vivo experiments were conducted in experimental albino rats. The study of cytotoxicity included kinetics of the fungal cell division and colony formation in the growth medium around the embedded alloy preparation. The study of genotoxicity included mutagenicity of the alloys in direct contact with the cells, and the micronucleus test. Results were tested by the analysis of variance. The study of cytotoxicity and genotoxicity showed minor differences between the alloys, which did not reach statistical significance in either in vitro or in vivo experiments. The risk of the use of various alloys in dentistry and a controversy about their biocompatibility and choice of experimental models, appear to justify further studies in the field.

Keywords

dental alloys; citotoxicity; genotoxicity

Hrčak ID:

99412

URI

https://hrcak.srce.hr/99412

Publication date:

15.9.1994.

Article data in other languages: croatian

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