Original scientific paper

Optimization of Chromatographic Conditions in Thin Layer Chromatography of Flavonoids and Phenolic Acids

Marica Medić-Šarić ; Faculty of Pharmacy and Biochemistry, University of Zagreb, A. Kovačića 1, 10000 Zagreb, Croatia
Ivona Jasprica ; Faculty of Pharmacy and Biochemistry, University of Zagreb, A. Kovačića 1, 10000 Zagreb, Croatia
Asja Smolčić-Bubalo ; Faculty of Pharmacy and Biochemistry, University of Zagreb, A. Kovačića 1, 10000 Zagreb, Croatia
Ana Mornar ; Faculty of Pharmacy and Biochemistry, University of Zagreb, A. Kovačića 1, 10000 Zagreb, Croatia

Abstract

Flavonoids are one of the largest groups of natural compounds known. They are supposed to have numerous physiological activities. There are many foods that contain flavonoids, but one of the most important sources of flavonoids is propolis. Besides flavonoids, phenolic acids are the main active substances of propolis. The list of uses and preparations of propolis is almost endless and demands an accurate analytical method to define the substances in this natural product. It can be easily analyzed by chromatographic methods, but before testing a new type of propolis it is opportune to optimize chromatographic conditions. The aim of this study is to optimize the chromatographic conditions in TLC of flavonoids and phenolic acids, as standard compounds that may be present in Croatian propolis. We compared 9 different mobile phases, using information theory and numerical taxonomy methods and applying the computer search program KT1, to find the most appropriate mobile phase, the optimal combination of two and three mobile phases for separation of standards.

Keywords

flavonoids; phenolic acids; optimization; TLC; information theory; numerical taxonomy

Hrčak ID:

102728

URI

https://hrcak.srce.hr/102728

Publication date:

31.5.2004.

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