Original scientific paper

https://doi.org/10.5599/jese.2013.0031

Electrochemical determination of an antitumour platinum(IV) complex: trans-[PtCl2(OH)2(dimethylamine)(isopro­pylamine)]. Application to biological samples

Carmen S. Hernandez Dominguez ; Departamento de Química Analítica y Análisis Instrumental, Facultad de Ciencias, Universidad Autónoma de Madrid, Cantoblanco, 28029 Madrid, Spain
Pedro Hernandez ; Departamento de Química Analítica y Análisis Instrumental, Facultad de Ciencias, Universidad Autónoma de Madrid, Cantoblanco, 28029 Madrid, Spain

Abstract

A differential pulse voltammetry (DPV) method has been applied for the first time for determination of trans-Pt[Cl2(OH)2(dimethylamine)(isopropylamine)]. To this end, all chemical and instrumental variables affecting the determination of trans-Pt[Cl2(OH)2(dimethylamine)(isopropylamine)] were optimized. From studies of the mechanisms governing the electrochemical response of trans-Pt[Cl2(OH)2(dimethylamine)(isopropylamine)], it was concluded that it is an electrochemically irreversible system with a reduc­tion under diffusion control, with a reduction potential of -425 mV. Under optimal conditions, the variation in the analytical signal (Ip) with trans-Pt[Cl2(OH)2(dimethylamine)(isopropylamine)] concentration is linear in the 0.8 µg mL-1 to 20 µg mL-1 range, with an LOD of 97 ng mL-1 and a LOQ of 323 ng mL-1, RSD = 1.58 % and Er = 0.83 %. The optimized method was applied to the determination of trans-Pt[Cl2(OH)2(di-methylamine)(isopropylamine)] in biological fluids, human urine and synthetic urine

Keywords

Trans-Pt[Cl2(OH)2(dimethylamine)(isopropylamine)]; differential pulse voltammetry; biological fluids; human urine

Hrčak ID:

103164

URI

https://hrcak.srce.hr/103164

Publication date:

19.4.2013.

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