Original scientific paper
https://doi.org/10.2478/10004-1254-64-2013-2319
Mutagenicity and DNA Damage of Bisphenol A and Its Structural Analogues in HepG2 Cells
Anja Fic
; Faculty of Pharmacy, University of Ljubljana, Ljubljana, Slovenia
Marija Sollner Dolenc
; Faculty of Pharmacy, University of Ljubljana, Ljubljana, Slovenia
Metka Filipič
; National Institute of Biology, Department for Genetic Toxicology and Cancer Biology, Ljubljana, Slovenia
Lucija Peterlin Mašić
; Faculty of Pharmacy, University of Ljubljana, Ljubljana, Slovenia
Abstract
Environmental oestrogen bisphenol A (BPA) and its analogues are widespread in our living environment. Because their production and use are increasing, exposure of humans to bisphenols is becoming a significant issue. We evaluated the mutagenic and genotoxic potential of eight BPA structural analogues (BPF, BPAF, BPZ, BPS, DMBPA, DMBPS, BP-1, and BP-2) using the Ames and comet assay, respectively. None of the tested bisphenols showed a mutagenic effect in Salmonella typhimurium strains TA98 and TA100 in either the presence or absence of external S9-mediated metabolic activation (Aroclor 1254-induced male rat liver). Potential genotoxicity of bisphenols was determined in the human hepatoma cell line (HepG2) at non-cytotoxic concentrations (0.1 μmol L-1 to 10 μmol L-1) after 4-hour and 24-hour exposure. In the comet assay, BPA and its analogue BPS induced signifi cant DNA damage only after the 24-hour exposure, while analogues DMBPS, BP-1, and BP-2 induced a transient increase in DNA strand breaks observed only after the 4-hour exposure. BPF, BPAF, BPZ, and DMBPA did not induce DNA damage.
Keywords
Ames test; bisphenols; comet assay; genotoxicity
Hrčak ID:
103924
URI
Publication date:
18.6.2013.
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