Original scientific paper
Efficiencies of Growth and Angiostatin Expression in Cultures ofPichia pastoris FedwithMixed Carbon Sources
J. Xie
; Department of Food Science and Technology, School of Biotechnology, East China University of Science and Technology, Shanghai 200237, China
R. Yang
; State Key Laboratory of Bioreactor Engineering
Q. Zhou
; Institute of Biochemistry and Cell Biology, Shanghai Institute of Biological Science, Chinese Academy of Science, Shanghai 200031, China
P. Du
; Institute of Biochemistry and Cell Biology, Shanghai Institute of Biological Science, Chinese Academy of Science, Shanghai 200031, China
R. Gan
; Institute of Biochemistry and Cell Biology, Shanghai Institute of Biological Science, Chinese Academy of Science, Shanghai 200031, China
Q. Ye
; State Key Laboratory of Bioreactor Engineering
Abstract
Different carbon sources, glycerol, sorbitol, acetate and lactate were added together with methanol in the induction phase of Pichia pastoris culture for angiostatin production in a 5-L fermentor. Mass balances were carried out for different processes, yield coefficients
were discussed and stoichiometric coefficients were estimated to compare the efficiency of different carbon sources. Glycerol had the highest reduction degree among the four non-methanol carbon sources, as well as higher energetic yield coefficient for biomass production () and lower respiratory quotient (RQ), indicating that glycerol was a more efficient carbon source for biomass formation than the other three non-methanol carbon sources. Lactic acid, a non-repression carbon sources, had lower reduction degree,
but achieved the highest energetic yield coefficient for angiostatin production (P).
Keywords
Mass balance; Pichia pastoris; glycerol; lactate; angiostatin
Hrčak ID:
104788
URI
Publication date:
1.7.2013.
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